A common assembly module in injectisome and flagellar type III secretion sorting platforms

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A common assembly module in injectisome and flagellar type III secretion sorting platforms

Translocating proteins across the double membrane of Gram-negative bacteria, type III secretion systems (T3SS) occur in two evolutionarily related forms: injectisomes, delivering virulence factors into host cells, and the flagellar system, secreting the polymeric filament used for motility. While both systems share related elements of a cytoplasmic sorting platform that facilitates the hierarch...

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The flagellum and the injectisome are two of the most complex and fascinating bacterial nanomachines. At their core, they share a type III secretion system (T3SS), a transmembrane export complex that forms the extracellular appendages, the flagellar filament and the injectisome needle. Recent advances, combining structural biology, cryo-electron tomography, molecular genetics, in vivo imaging, ...

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Under certain conditions, pathogens like Yersinia secrete high levels of proteins. This ability correlates with cytotoxicity and virulence. The complete inactivity of the secreted proteins when added directly to host cells stimulated research to disclose this ‘‘non-AB toxin mechanism’’ of virulence, and eventually led to the discovery of the type III secretion mechanism and the ‘‘injection mode...

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With the rise of multidrug resistance, Pseudomonas aeruginosa infections require alternative therapeutics. The injectisome (iT3SS) and flagellar (fT3SS) type III secretion systems are 2 virulence factors associated with poor clinical outcomes. iT3SS translocates toxins, rod, needle, or regulator proteins, and flagellin into the host cell cytoplasm and causes cytotoxicity and NLRC4-dependent inf...

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ژورنال

عنوان ژورنال: Nature Communications

سال: 2015

ISSN: 2041-1723

DOI: 10.1038/ncomms8125